A RESEARCHER HAS SYNCHRONIZED TWO DIFFERENT E. COLI CULTURES SO THAT ALL OF THE CELLS ENTER CELL DIVISION SIMULTANEOUSLY. ONE CULTURE IS WILD-TYPE E. COLI CELLS, AND THE OTHER CULTURE IS A MUTANT E.

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A RESEARCHER HAS SYNCHRONIZED TWO DIFFERENT E. COLI CULTURES SO THAT ALL OF THE CELLS ENTER CELL DIVISION SIMULTANEOUSLY. ONE CULTURE IS WILD-TYPE E. COLI CELLS, AND THE OTHER CULTURE IS A MUTANT E.

COLI THAT DOES NOT HAVE THE GENE FOR DAM METHYLASE. HE ISOLATES THE DNA FROM BOTH WILD-TYPE AND MUTANT E. COLI 20 MINUTES AFTER REPLICATION IS COMPLETE. WHAT IS THE METHYLATION STATE FOR EACH STRAIN OF E. COLI? WHAT WOULD BE THE METHYLATION STATE FOR EACH BACTERIAL STRAIN IF YOU ISOLATED THE DNA IMMEDIATELY AFTER REPLICATION TERMINATION?

A researcher has synchronized two different E. coli cultures so that all of the cells enter cell division simultaneously. One culture is wild-type E. coli cells, and the other culture is a mutant E. coli that do not have the gene for Dam methylase. He isolates the DNA from both wild-type and mutant E. coli 20 minutes after replication is complete. What is the methylation state for each strain of E. coli? What would be the methylation state for each bacterial strain if you isolated the DNA immediately after replication termination?

 

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